Increasing the Throughput of Ligandable Cysteine Hotspots Profiling by Mass Spectrometry to Guide RAS Pathway Drug Discovery

Time: 2:00 pm
day: Day Two


  • Hotspots are accessible, reactive and ligandable residues that can serve as covalent drug targets
  • Systematic cataloging of the hotspots present in a cellular proteome is instrumental for advancements in drug discovery
  • We implemented sample hyperplexing into an activity-based proteome profiling (ABPP) workflow to increase the depth and throughput of ligandable cysteine hotspots identification by mass spectrometry